N-ethyl-N-nitrosourea (ENU) mutagenesis and male fertility research
نویسندگان
چکیده
منابع مشابه
N-ethyl-N-nitrosourea mutagenesis: boarding the mouse mutant express.
In the mouse, random mutagenesis with N-ethyl-N-nitrosourea (ENU) has been used since the 1970s in forward mutagenesis screens. However, only in the last decade has ENU mutagenesis been harnessed to generate a myriad of new mouse mutations in large-scale genetic screens and focused, smaller efforts. The development of additional genetic tools, such as balancer chromosomes, refinements in geneti...
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Metazoan introns contain a polypyrimidine tract immediately upstream of the AG dinucleotide that defines the 3' splice site. In the nematode Caenorhabditis elegans, 3' splice sites are characterized by a highly conserved UUUUCAG/R octamer motif. While the conservation of pyrimidines in this motif is strongly suggestive of their importance in pre-mRNA splicing, in vivo evidence in support of thi...
متن کاملInduced by N-ethyl-N-nitrosourea
Phenotypic reversion of the rubber-band, muscledefective phenotype conferred by unc-93(e1500) was used to determine the utility of Nethyl-Nnitrosourea (ENU) as a mutagen for genetic research in Cuenmhabditis ekguns. In this system, ENU produces revertants at a frequency of 3 X equivalent to that of the commonly used mutagen, EMS. The gene identity of 154 ENU-induced revertants shows that the di...
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The primary role of the mismatch repair (MMR) system is the avoidance of mutations caused by replication and recombination errors. Furthermore, the lethality of methylating agents has been attributed to the processing of O(6)-methylguanine lesions in DNA by MMR. Loss of the MSH2 protein completely abolishes repair function and results in reduced cell killing by methylating agents and accelerate...
متن کاملAn N-ethyl-N-nitrosourea mutagenesis screen for epigenetic mutations in the mouse.
The mammalian epigenetic phenomena of X inactivation and genomic imprinting are incompletely understood. X inactivation equalizes X-linked expression between males and females by silencing genes on one X chromosome during female embryogenesis. Genomic imprinting functionally distinguishes the parental genomes, resulting in parent-specific monoallelic expression of particular genes. N-ethyl-N-ni...
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ژورنال
عنوان ژورنال: Human Reproduction Update
سال: 2006
ISSN: 1460-2369,1355-4786
DOI: 10.1093/humupd/dmk004